ABSTRACT
The antilisterial properties of some vegetables such as Daucus carota have already been reported, but there is no report on Zardak [Parstinaca sativa]. The objective of this study was comparison of the inhibitory effect of Zardak and Daucus carota [shredded and concentrations of juice] on two suspensions of Listeria [L.] monocytogenes. In this experimental study, contamination of two carrot cultivars was examined according to national standard no. 8035-1. Then carrot treatments prepared. Two suspensions [10[3] and 10[5] CFU/ml] of L. monocytogenes serotype 1295 PTCC inoculated on shredded and concentrations of carrot juices. After 1 and 24 h in refrigerator, inoculations were cultured on Listeria Selective Agar. The results reported according to the difference between the typical Listeria colonies on inoculated and control plates. Data were analyzed by Kruskal-Wallis test. P-Values lower than 0.05 was considered significant. Carrot samples had no Listeria contamination. Statistical analysis showed both cultivars had significant inhibitory effect on L. monocytogenes, but it is more in Zardak than Daucus carota [p<0.005]. The inhibitory effect of Zardak was shown on both bacterial concentrations, but Daucus carota effect only on 10[3] CFU/ml. The effect of Zardak became more after 24 h in refrigerator than 1 h [p<0.05], but it made no difference in Daucus carota. Whole carrot juice had more effect than other treatments [p<0.05]. We noticed fresh carrots had no inhibitory effect, but it could be seen by keeping carrots in refrigerated or room temperature for 4 days. Both Zardak and Daucus carota have antilisterial properties, but the effect of Zardak was mere significant. Variable inhibitory conditions would be objectives of other researches. On the other hand, probability of extracting antilisterial compounds from Zardak and using as a natural food preservative is suggested
Subject(s)
Daucus carota , Pastinaca , Anti-Infective AgentsABSTRACT
Complications of pelvic irradiation have gained more attention because of increased survival of patients. CO-60 Teletherapy has wide penumbra in comparison with linear accelerator that can cause testes irradiation during radiotherapy in vicinity of lower border of pelvic portal when treated for rectal cancer. In this study we compared testicular doses of testes when pelvis is irradiated for rectal cancer and its effect on sex hormone levels. In a cohort study, rectal cancer patients treated by pelvic irradiation concomitant with chemotherapy in two group. A group treated by Co-60 Teletherapy and another group by Linear Accelerator [LINAC]. Sex hormones serum levels were measured immediately before and 3 to 6 weeks after finishing irradiation. Testicular doses were measured by TLD [LiF], 3 times during whole course of irradiation in 5 patients of each group. T test and Mann-Whitney were used to compare data. 28 patients entered in study but 2 patients died early in course of radiotherapy [1 in each group], and one patient excluded because testes was inside of treatment portals. Patients and disease characteristics were similar between groups. Testes doses in patients treated by LINAC [55 +/- 24.7 mGy] was significantly lower than Co-60 [120 +/- 23 mGy] [p <0.001]. FSH and LH serumic level increased after irradiation in both groups and there is not a relation between FSH and LH levels with treatment machine [p<0.2] for LINAC and p<0.6 for Co-60. Decrease in serumic levels of testosterone was significant in patients treated by CO-60 [p<0.05], but was not significant in LINAC group [p<0.3]. It seems using LINAC in treating patient with rectal cancer can decrease testes doses but can not prevent hormonal changes. We suggest extra shield to decrease testes doses below the toxic dose